Listed below are the major equipment available.  Where applicable, links to Google calendars are included for lab members to sign up for time on the equipment.  Others wishing to use equipment should contact Daniela Fera
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Main Laboratory (SCI 293)

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Mosquito Crystallization Robot ​

Sets up trays in 96-well format; can dispense from crystal screens (as low as 100nL for dry transfer) as well additive screens (down to 15nL for wet-transfer); can set dispense up to 4 drops per well.
All users must be trained by Daniela Fera.
Instructions and Video
​Pre-run Questionnaire (complete before requesting training)
Calendar

Stereomicroscope
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Can be used for looking at crystal trays set up with the Mosquito or manually.
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EM Glow Discharger
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Makes EM grids hydrophilic, which is necessary before putting protein sample on the grids. Used for negative stain and cryo-EM studies.​

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Bacterial Incubator/Shaker

Infors HT Multitron Standard - Can shake up to 12 two-liter flasks at variable temperatures (above and below room temperature). 
​Calendar

Microcentrifuge

Non-refrigerated. Can hold and spin up to 24 eppendorf tubes.

Refrigerated Centrifuge

Adaptors available for spinning 15- and 50-mL falcon tubes.

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NanoDrop One

UV-Vis Spectrophotometer. Used to obtain DNA and protein sample concentrations. Requires only 2uL of sample, or larger volumes if a cuvette is used.

BLItz

Measures binding kinetics; requires only 4uL of sample.
Calendar

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AKTA Purifier FPLC

High quality protein purification.  Capable of both ion exchange and size exclusion chromatography.
Calendar
Instructions

AKTA Pure FPLC

High quality protein purification.  Capable of both ion exchange and size exclusion chromatography. Can connect multiple columns simultaneously.
Calendar
Instructions

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DNA Gel Electrophoresis Chamber

Runs agarose gels. Separates DNA strands by size.

Protein Gel Electrophoresis Chamber

Runs polyacrylamide gels (up to 4 simultaneously). Separates protein bands by size.

Thermal Cycler
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Performs standard PCR.  Programmable for other functions as well.
Calendar

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Shipper Dewar

Used for shipping crystals and/or cryo-EM grids frozen in LN2. Can be used for shipping other frozen things as well.

Crystal Pucks and Sleeve

For shipment of frozen crystals. Each puck can hold 16 crystals. 7 pucks are available. The sleeve holds the pucks in place.

Cryo-EM Pucks

For shipment of frozen electron microscopy grids. Each puck can hold 12 grid boxes and each grid box can hold 4 grids. 2 pucks are available. The same sleeve as for crystal pucks can be used.

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BSL2 Laboratory (SCI 166)
Note: all users of this lab must be trained by Daniela Fera

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Biosafety Cabinets

Two are available; provide sterile environment for tissue culture maintenance.
Instructions

Calendar (left cabinet)
Calendar (right cabinet)

Cell Countess II

Automated cell counter.

Centrifuge

Non-refrigerated. Offers the ability to spin 15- and 50-mL samples tubes.  Microplates can be spun as well, using appropriate adaptors.

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CO2 Incubator

For mammalian tissue culture growth.  Two shakers are available for growing culture in suspension.  A shelf is available for adherent cell growth.

Liquid Nitrogen Box Dewar

Storage for mammalian cells.  Holds 25 boxes, each with 25 slots for 2mL cryo-tubes.

Microscope
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Can be used for viewing adherent cell cultures or for counting cells.

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